How to Use a Centrifuge DL6MB to Prepare Pharmaceutical Protein
Using the Optima XPN Ultracentrifuge DL6MB to prepare pharmaceutical protein in High Speed Centrifuge has several advantages over other methods. Aside from producing more yield and faster speeds, this centrifuge can handle higher loads of protein. This makes it an ideal choice for laboratories looking to expand their capacity. Moreover, it can be used in a wide variety of applications, from high-speed separations to density gradient centrifugation.
Optima XPN Ultracentrifuge DL6MB
Optima XP's DL6MB is a step above your standard lab set up, and that's a good thing. It's a hefty price tag, but you'll get your money's worth in the form of a top-of-the-line performance. The DL6MB is built with quality components spanning from its helms to its wheels. It also offers a plethora of standard and customizing options. Among the most notable features are the optional g-Max for maximum volume efficiency and Quick-Seal for biocontainment. The L-80 XP's air-cooled design is also noteworthy. The DL6MB can run at speeds of up to 100,000 rpm and can accommodate tubes filled at least 10 percent over or under. The DL6MB is a worthy adversary in the field of ultracentrifuges. It is the most intelligent of the lot and a well-oiled machine.
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Density gradient centrifugation
Using density gradient centrifugation for pharmaceutical protein preparation is not a new concept. It has been used by biochemists and biophysicists for years. In fact, the technique has become quite popular in recent years.
The basic idea of density gradient centrifugation like in Floor Stand Low Speed Centrifuge is to create a fluid transition that causes the particles in suspension to move to a higher density and the particles at the top to move to a lower density. There are two main types of density gradients. The first is a discontinuous gradient and the other is a step gradient.
An example of a discontinuous gradient is a 10-40% sucrose gradient. This is made by layering diluted whole blood onto a high density medium.
The density of the solution is equal to the mass:volume ratio. It is difficult to get a good density difference without mixing. This is because the particles in the suspended liquid are individually denser than the liquid at the top of the gradient.
Isopycnic density gradients are more steep. This means that the mass of the particles per gradient length is greater. These gradients also support a larger quantity of samples.
This type of gradient is useful for resolving heterogeneous mixtures of particles. A special device called a gradient maker is required to make this type of gradient. A gradient maker is a device that can pipette individual gradient steps into a centrifuge tube.
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Maintenance of a centrifuge
Whether you own a centrifuge DL6MB to prepare pharmaceutical protein or you just purchased one, you need to know how to maintain it properly. With a few simple steps, you can prevent damage and keep your instrument in good working condition.
The rotor of your centrifuge needs to be checked regularly. It should be replaced at the first signs of cracking or brittleness. It is also important to balance the tubes as they spin. If there is a misbalance, the centrifuge can become dangerous, and other laboratory personnel can be injured.
The rotor chamber should be cleaned using a damp cloth. Be careful not to use abrasives, as this may damage coatings. If you do need to use abrasives, be sure to clean the parts in a neutral detergent. This will protect the rotor from contamination from residual chemicals.
For regular cleaning, you should always follow the manufacturer's instructions for Mini Centrifuge. If you cannot find the instructions, contact your service representative. They may have a copy of the manual, or you can search online.
After each run, the interior of your centrifuge should be cleaned. This includes the specimen holder, supports, and rotor. If the rotor or specimen holder are made of plastic, you should autoclave them. You should also clean the touchscreens.
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