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The Use of Acetonitrile in Gas Chromatography-Mass Spectrometry Analysis

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Anjali Pawar
The Use of Acetonitrile in Gas Chromatography-Mass Spectrometry Analysis

Acetonitrile is widely used as a mobile phase solvent in gas chromatography-mass spectrometry (GC-MS) analysis due to its low viscosity, low volatility, and high polarity. It is commonly used to separate and analyze small molecules, such as amino acids, peptides, and fatty acids. One of the advantages of using acetonitrile in natural product extraction is its ability to extract polar and nonpolar compounds. This makes it a versatile solvent for the extraction of a wide range of natural products.


In GC-MS analysis, the Acetonitrile is typically used in a mixture with other solvents, such as methanol or water. The ratio of solvents used in the mixture can affect the separation and detection of analytes in the sample. However, it is important to note that the use of acetonitrile in natural product extraction can also result in the extraction of unwanted compounds, such as impurities or toxic substances. Therefore, careful consideration must be given to the selection of solvents and extraction conditions to ensure the safety and purity of the extracted natural products.


One of the benefits of using acetonitrile in GC-MS analysis is that it can help to reduce the background noise in the mass spectrometer. Acetonitrile has a lower ionization potential compared to other solvents, which results in fewer background ions in the mass spectrometer. It is also important to note that the use of high-purity water is essential in the purification of acetonitrile. Water can react with acetonitrile to form impurities, and therefore, the water used in HPLC systems should be purified using reverse osmosis or deionization techniques.



Read more @ http://sparkblog45.weebly.com/article/acetonitrile-used-as-a-solvent-in-purification-of-butadiene


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